NUST Institutions Library Catalogue

Tracking of Novel Plasmid mediated Azithromycin's resistant & virulence genes in Salmonella Typhi / (Record no. 609121)

000 -LEADER
fixed length control field 02699nam a22001577a 4500
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 610
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Gul, Sania
245 ## - TITLE STATEMENT
Title Tracking of Novel Plasmid mediated Azithromycin's resistant & virulence genes in Salmonella Typhi /
Statement of responsibility, etc. Sania Gul
264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Islamabad :
Name of producer, publisher, distributor, manufacturer SMME- NUST;
Date of production, publication, distribution, manufacture, or copyright notice 2022.
300 ## - PHYSICAL DESCRIPTION
Extent 48p.
Other physical details Soft Copy
Dimensions 30cm
500 ## - GENERAL NOTE
General note Typhoid fever is a serious public health threat for which the needs of antimicrobial treatment are<br/>increasing. This acute illness coupled with fever caused by the virulent and pathogenic multi-drug<br/>resistance plus extensively-drug resistant salmonella enterica typhi bacteria. A total of 85 fresh<br/>human clinical Salmonella Enterica Serovar typhi isolates were recovered from patients in<br/>different hospitals of Pakistan during the period of 2020-2021. Of the total 85 samples, 35 (40.4%)<br/>strains were phenotypic azithromycin-resistant while, 24 (28.5%) were genotypic azithromycinresistant strains of S.typhi. The PCR analysis publicized that 59 isolates (69.5%) contained acrB<br/>gene expression and 46 isolates (54.7%) carried rplV gene expression and all the isolates carried<br/>rplV gene expression were also positive for acrB gene. Similarly, the virulotyping of three<br/>specified genes such as; pefA, sopE1 and gipA by polymerase chain reaction (PCR) of salmonella<br/>typhi isolates was also done in this study. By using Pearson’s chi-square test, a significance<br/>differences were observed between MDR and XDR status with specified virulence genes.<br/>However, no significant differences were identified among any virulence gene and antibiotic<br/>resistance patterns at less than p value (p<.05). In addition, the final product of two-way factorial<br/>ANOVA identifies significant differences between both factors i.e., virulence genes and AR<br/>patterns, with F calculated is bigger than F-critical value. However, the interaction effect between<br/>virulent and AR-status was not significant, as F-calculated was smaller (1.014) than F-critical<br/>(3.84). This study is designed to determine whether there is a complex relationship exists between<br/>AR status and virulence genes of S-typhi. However, the study of rplV gene expression is Novel in<br/>Pakistan and is conducting for the first time in this study. The findings from this study will help<br/>the scientific community to permit a better perceptive of pathogenic character of salmonella typhi<br/>and to develop a modified-treatment strategy that will help in the management, cure and prevention<br/>from salmonellosis.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MS Biomedical Sciences (BMS)
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Supervisor : Dr. Amer Sohail Kashif
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://10.250.8.41:8080/xmlui/handle/123456789/30868">http://10.250.8.41:8080/xmlui/handle/123456789/30868</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Koha item type Thesis
Holdings
Withdrawn status Permanent Location Current Location Shelving location Date acquired Full call number Barcode Koha item type
  School of Mechanical & Manufacturing Engineering (SMME) School of Mechanical & Manufacturing Engineering (SMME) E-Books 05/15/2024 610 SMME-TH-728 Thesis
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