Prevalence and Mechanism of Carbapenem-resistant Genes in Acinetobacter baumannii / (Record no. 609122)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02606nam a22001577a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 610 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Khalid, Komal |
| 245 ## - TITLE STATEMENT | |
| Title | Prevalence and Mechanism of Carbapenem-resistant Genes in Acinetobacter baumannii / |
| Statement of responsibility, etc. | Komal Khalid |
| 264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Islamabad : |
| Name of producer, publisher, distributor, manufacturer | SMME- NUST; |
| Date of production, publication, distribution, manufacture, or copyright notice | 2022. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 58p. |
| Other physical details | Soft Copy |
| Dimensions | 30cm |
| 500 ## - GENERAL NOTE | |
| General note | Coccobacilli with a gram-negative Acinetobacter baumannii is a member of the ESKAPE group<br/>of free-living organisms, which are widely found in the environment in places like water, food,<br/>soil, sewage, animals, and humans. It is an opportunistic pathogen that has been implicated in<br/>numerous nosocomial infections across the globe. Carbapenem resistance has formed into a<br/>potential threat to humanity because of its rising prevalence among healthcare facilities. The<br/>present study was designed to characterize the presence of carbapenem-resistant genes including<br/>multidrug-resistant Acinetobacter baumannii strains, isolated from the tertiary care hospital in<br/>Pakistan. A biochemical test was performed for the initial identification of bacterial strains and<br/>further confirmed by MALDI-TOF mass spectrometry. Antibiotic susceptibility testing was done<br/>by using the standard Kirby-Bauer disk diffusion method, and carbapenem resistance determinants<br/>were further screened Metallo-beta-lactamase manufacture through Imipenem/Imipenem-EDTACDT. All 48 A. baumannii strains were further amplified by PCR for detection of OXA-51-like,<br/>OXA-23-like, and blaNDM genes. The OXA-51-like gene was detected in 46 strains (95.83%),<br/>while OXA-23-like was detected in 44 (91.66%) strains of carbapenem-resistant Acinetobacter<br/>baumannii. Moreover, New Dehli MBL, blaNDM gene was identified in four (8.33%) MBL<br/>positive carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. All 4 positive blaNDM<br/>isolates were co-expressed by the OXA-23-like gene while OXA-51-like was absent in one<br/>blaNDM positive strain of carbapenem-resistant Acinetobacter baumannii isolates. A high<br/>prevalence of OXA-51-like, and OXA-23-like genes was seen among the carbapenem-resistant<br/>Acinetobacter baumannii isolates, followed by blaNDM1. The results of the study revealed that<br/>MDR Acinetobacter baumannii strains from two important cities in Pakistan (Rawalpindi and<br/>Islamabad) had a significant presence of carbapenem-resistant genes. This finding may result in<br/>clinical therapeutic failures. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MS Biomedical Sciences (BMS) |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Supervisor : Dr. Amer Sohail Kashif |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://10.250.8.41:8080/xmlui/handle/123456789/30869">http://10.250.8.41:8080/xmlui/handle/123456789/30869</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Thesis |
| Withdrawn status | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Koha item type |
|---|---|---|---|---|---|---|---|
| School of Mechanical & Manufacturing Engineering (SMME) | School of Mechanical & Manufacturing Engineering (SMME) | E-Books | 05/15/2024 | 610 | SMME-TH-727 | Thesis |