Therapeutic Application of Crude Mitochondrial Transplantation for Diabetic Wound Healing / (Record no. 614596)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02221nam a22001577a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 610 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hameed, Farhan |
| 245 ## - TITLE STATEMENT | |
| Title | Therapeutic Application of Crude Mitochondrial Transplantation for Diabetic Wound Healing / |
| Statement of responsibility, etc. | Farhan Hameed |
| 264 ## - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Islamabad : |
| Name of producer, publisher, distributor, manufacturer | SMME- NUST; |
| Date of production, publication, distribution, manufacture, or copyright notice | 2025. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 77p. |
| Other physical details | Soft Copy |
| Dimensions | 30cm |
| 500 ## - GENERAL NOTE | |
| General note | Chronic wounds in diabetic condition pose a persistent clinical challenge, primarily due<br/>to impaired cellular energy metabolism and delayed tissue regeneration. Mitochondrial<br/>dysfunction plays pivotal role in this impaired healing process, making mitochondrial<br/>transplantation (MT) a promising therapeutic strategy. This study investigates the<br/>efficacy of MT in accelerating wound repair using a diabetic mice model. Full-thickness<br/>excisional wounds (6mm) were created on the dorsal surface of diabetic mice, followed<br/>by subcutaneous administration of isolated crude mitochondria at low, moderate and<br/>high dose (5ug, 10ug, and 20ug) mitochondria per wound. A vehicle-treated group<br/>receiving phosphate-buffered saline (PBS) served as the control.<br/>Mitochondrial viability and quantification were assessed using MTT assay and Bradford<br/>in wound contraction. Wound closure analysis revealed a dose-dependent acceleration of<br/>healing, with high-dose group exhibiting the most significant improvement in wound<br/>contraction and re-epithelialization. Histological analysis confirmed enhanced tissue<br/>regeneration in the treatment groups. Furthermore real-time PCR analysis demonstrated a<br/>significant upregulation of RAC1, CDC42, and VEGF in the moderate and high treatment<br/>groups, indicating enhanced cytoskeletal remodeling, cellular migration and<br/>angiogenesis.<br/>These findings highlight the therapeutic potential of crude mitochondrial transplantation<br/>in diabetic wound healing by restoring cellular bioenergetics and promoting tissue repair.<br/>This approach offers a promising regenerative strategy for managing chronic wound and<br/>improving diabetic wound outcomes. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MS Biomedical Sciences (BMS) |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Supervisor : Dr. Hussain Mustatab Wahedi |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://10.250.8.41:8080/xmlui/handle/123456789/54551">http://10.250.8.41:8080/xmlui/handle/123456789/54551</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Thesis |
| Withdrawn status | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Koha item type |
|---|---|---|---|---|---|---|---|
| School of Mechanical & Manufacturing Engineering (SMME) | School of Mechanical & Manufacturing Engineering (SMME) | E-Books | 08/29/2025 | 610 | SMME-TH-1152 | Thesis |