NUST Institutions Library Catalogue

Hypochromic erythrocytes are red blood cells (RBC) with central pallor larger than
normal and hence overall appears paler. This paleness is due to lack of hemoglobin
responsible for carrying oxygen and giving red color to erythrocytes. Hypochromic erythrocytes are commonly associated with iron deficiency anemia. Anemia is manually
diagnosed through peripheral blood smear (PBS) test. The monolayer of blood smear
is observed by pathologist as its the only region with erythrocytes is observably visible.
PBS test is the gold standard in analyzing blood cells yet its time consuming tedious
task. Automated techniques are applied to analyze the cells but did not properly addressed the overlapped anemia cells. Overlapped cells are also overlooked by pathologist
as well in manual PBS test. Trigonometric projection is mapped from the detected enclosed edges of cells from microscopic images. Prior to edge detection, the images are
color processed to remove all leukocytes and platelets leaving only Erythrocytes and
later eroded and dilated to remove small residue preserving the cells. The proposed
method performance metric is 95.27 %, 82.45% and 88.39% for precision, recall and F1
score respectively.