Basic techniques in molecular biology /
Stefan Surzycki.
- Berlin ; New York : Springer, c2000.
- viii, 434 p. : ill. ; 25 cm.
- Springer lab manual .
Includes bibliographical references and index.
General Aspects of DNA Isolation and Purification -- Preparation of Genomic DNA from Animal Cells -- Preparation of Genomic DNA from Plant Cells -- Preparation of Genomic DNA from Bacteria -- Isolation of Plasmid DNA -- Isolation and Purification of RNA -- Isolation of PolyA+ RNA -- Agarose Gel Electrophoresis of DNA -- Preparation of Probes for Hybridization -- Nucleic Acid Hybridization. A Theoretical Consideration -- DNA Transfer and Hybridization -- Northern Transfer and Hybridization -- DNA Cloning. General Consideration -- DNA Cloning - Experimental Procedures -- DNA Sequencing -- PCR Analysis