Evaluating the Synergistic Role of Insulin and Photobiomodulation in alleviating Diabetic Neuropathy and Cognitive Impairment /
Hooreen Kayani
- 88p. Soft Copy 30cm
Diabetic neuropathy and cognitive impairment are debilitating complications of diabetes, yet effective multi-targeted therapies remain limited. This study investigated the synergistic effects of Insulin and Photobiomodulation (PBM) in a streptozotocin (STZ) induced diabetic mouse model. Mice were divided into five groups: healthy control, diseased, PBM-treated, Insulintreated, and PBM + Insulin-treated, and received treatments for seven days. Functional outcomes were assessed through behavioural and biochemical analyses, while histopathological and molecular evaluations examined tissue integrity and the expression of neuroprotective markers, including Brain-derived Neurotrophic Factor (BDNF) and Myelin Protein Zero (MPZ), with β-actin as a reference. The combination of PBM and Insulin produced the most pronounced improvements, demonstrating enhanced cognitive performance, reduced neuropathic pain, and preservation of neuronal structure, peripheral nerve integrity, and multi-organ morphology, including the brain, sciatic nerve, heart, liver, kidney, and lungs. PBM or Insulin alone provided partial protection, with moderate amelioration of functional deficits and tissue pathology. Mechanistically, combination therapy upregulated BDNF and MPZ expression, suggesting a molecular basis for enhanced neuroprotection and remyelination. These findings highlight the potential of PBM combined with Insulin as a synergistic intervention to mitigate diabetes-induced neuropathy and cognitive decline. Incorporating such multi-modal strategies may offer a promising avenue for managing diabetes-related multi-organ complications and improving overall outcomes in diabetic patients.