03155nam a22001697a 4500082000800000100002800008245009600036264003800132300005000170500250500220650003602725700004102761856005702802942001302859999001902872952009402891  a610  aPerveen, Anita 9133977  aPsoriasis & Neuroinflammation: Exploring Cytokine Release in Barrier and Metabolic Organs /  aIslamabad : bSMME- NUST; c2026.  a99p.bIslamabad : SMME- NUST; Soft Copyc30cm  aPsoriasis is a chronic systemic inflammatory disease with its effects beyond skin. One
major comorbidity reported to be highly prevalent in psoriatic patients is cognitive
impairment. Moreover, overexpression of pro-inflammatory cytokines plays a central role
in development and progression of psoriasis and neuroinflammation. This study aims to
explore the role of inflammatory cytokines in neurodegeneration and disease progression
in psoriatic patients. Furthermore, it assesses the therapeutic effect of curcumin-chamomile
based plant extract loaded onto PVA nanoparticles via wet lab and bioinformatics tools.
Curcumin and Chamomile Plant extract-based drug was designed and loaded with Nanoparticles to use as treatment. The drug was tested on various parameters to check its toxicity
and reactivity before using in-vivo. Psoriatic animal model was designed by imiquimod
(IMQ) application on male BALB/c mice. The complete In-Vivo study from disease
induction to treatment followed by collection of cortex, hippocampus, Eyes, Skin and Liver
was done which were later used for gene expression analysis. Protein hallmarks of
neurodegeneration; α-Syn, and inflammatory cytokines like IL‐1β, TNF‐α, PEBP-1 and
HSP-90AB1 were measured at transcriptional level in cortex, hippocampus, Eyes, Skin
and Liver. Disease induction using IMQ resulted in excessive production of keratinocytes.
Pro-inflammatory cytokines were over-expressed whereas α-Syn showed a moderate
increase in hippocampus, eyes, cortex, liver and skin. Both treatment groups showed a
significant decline in α-Syn and normalization and downregulation of pro-inflammatory
cytokines. Molecular docking revealed potential interactions between proteins and
cytokines, and proteins and phytochemicals exhibiting potential roles in alleviating the
disease pathology. Pathway enrichment analysis was carried out to understand the
underlying mechanisms involved in disease progression and roles of these proteins. The
increased expression of inflammatory markers causes neuroinflammation and contribute to
depression and cognitive impairment, which can be reversed using the novel designed drug.
In psoriatic conditions, elevated levels of inflammatory cytokines cause chronic
inflammation, which trigger neuroinflammatory transcription factors. The Curcuminchamomile based novel drug encapsulated with nanoparticles comes as a new promising
approach to cure neurodegenerative diseases, and the comorbidities arising from psoriasis.   aMS Biomedical Sciences (BMS)     aSupervisor : Dr. Saima Zafar9122351  uhttp://10.250.8.41:8080/xmlui/handle/123456789/57525  2ddccTHE  c616895d616895  00104070aSMMEbSMMEcEBd2026-04-08l0o610pSMME-TH-1211r2026-04-08w2026-04-08yTHE