Effectivity of Rutin-bound Carbon dots in preventing the aggregation of Amyloid beta /

By Noor Afza. . 78p. , According to the World Health Organization up to 55 million people have dementia resulting in concerning socio-economic implications. About 60-80% cases of dementia are associated with Alzheimer's disease. With all the advances in medicine, there still is no cure. Blood-brain barrier monitors the entry and exit of nutrients and molecules which makes it difficult for various drugs and therapeutic molecules to cross it. Carbon dots are made from glucose to overcome the BBB. Rutin is a naturally existing flavonoid extracted from plants and has therapeutic effects in neuroprotection. This study aims to improve the effectiveness of rutin combined with targeted delivery of carbon dots. Carbon dots were loaded with Rutin to make CD-Rutin, a nano-sized combination with a targeted drug. FTIR, UV-IR, and SEM analysis have produced positive results regarding the doping of CDs with Rutin. Administration of CD-Rutin was done in AD-like rat models at eight to twelve months of age. Alzheimer's was induced in rats with Aluminum Chloride and D-galactose through IP administration for two weeks. Behavioral tests were performed to check the progression of the disease. In silico analysis was also done to check ligand-protein interaction to check variation in the binding of Aβ isoforms with Rutin. Afterward, a single injection of CD-Rutin (10 mg/kg) was given intraperitoneally as well. Behavioral testing was done after the administration of the drug. Characterization techniques revealed the successful formation of CDs and subsequent loading of Rutin onto the CDs resulting in a CD-Rutin combination. In silico analysis provided strong binding affinities of Rutin with Aβ isoforms affirming Rutin as a favorable treatment to target amyloid aggregates. 3D configuration showed binding of Rutin with hydrophobic domains of protein oligomers Behavioral testing provided significant difference in the treated group with better memory retention and performance in activities involved in behavioral testing. After behavioral testing, rats were dissected for molecular analysis including H&E to assess cell degeneration and Thioflavin T staining to assess amyloid aggregates in the brain tissues. Results provided positive data in terms of cell count in the cortex. Overall results suggest that memory impairment and cognitive abilities were significantly improved after injections. The results demonstrate the positive therapeutic potential of CD-Rutin in Alzheimer's treatment. 30cm.

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Therapeutic Potential of Rivastigmine for Managing Complications Associated with Ischemic Stroke /

By Abbasi, Irum Naeem . . 80p. , Stroke continues to be the world’s primary cause of morbidity and mortality, frequently with incapacitating consequences like movement dysfunction, cognitive decline, and neurological impairments. It is essential to manage stroke effectively to lessen its negative effects and enhance patient outcomes. The potential neuroprotective benefits of acetylcholinesterase inhibitors may assist maintain the integrity of brain tissue and lessen the damage that neurons may sustain after a stroke, making them a promising option in this situation. This comprehensive thesis explores the neuroprotective benefits of an acetylcholinesterase inhibitor, Rivastigmine, in relation to stroke outcomes in a variety of ways. Using an advanced integrative methodology, this study includes behavioral evaluations, in silico analysis, histopathological results, and molecular studies to give a comprehensive picture of the possible mechanisms behind therapeutic effects of Rivastigmine. Within the field of computer modelling, the docking interactions between SOD2 and TLR4, the target proteins of Rivastigmine, provide detailed structural information that directs further experimental validations. Following Rivastigmine treatment, the behavioral tests conducted exhibited improvements in cognitive, motor, and social domains. These results were further corroborated by histopathological analysis, which shows neuroprotective effects in Rivastigmine treated group as opposed to surgery (MCAO) group. Post Rivastigmine treatment, real-time PCR data showed a rise in SOD2 and a decrease in TLR4 levels in surgery (MCAO) rats, exhibiting antioxidant and anti-inflammatory effects of Rivastigmine. These findings provide interesting directions for future neuroprotective approaches and shed light on the possible therapeutic implications of Rivastigmine in reducing neurodegeneration that occurs in stroke. 30cm.

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Effectivity of Rutin-bound Carbon dots in preventing the aggregation of Amyloid beta /

Therapeutic Potential of Rivastigmine for Managing Complications Associated with Ischemic Stroke /