000			02283nam a22001577a 4500
082			_a610
100			_aKayani, Hooreen _9131226
245			_aEvaluating the Synergistic Role of Insulin and Photobiomodulation in alleviating Diabetic Neuropathy and Cognitive Impairment / _cHooreen Kayani
264			_aIslamabad : _bSMME- NUST; _c2025.
300			_a88p. _bSoft Copy _c30cm
500			_aDiabetic neuropathy and cognitive impairment are debilitating complications of diabetes, yet effective multi-targeted therapies remain limited. This study investigated the synergistic effects of Insulin and Photobiomodulation (PBM) in a streptozotocin (STZ) induced diabetic mouse model. Mice were divided into five groups: healthy control, diseased, PBM-treated, Insulintreated, and PBM + Insulin-treated, and received treatments for seven days. Functional outcomes were assessed through behavioural and biochemical analyses, while histopathological and molecular evaluations examined tissue integrity and the expression of neuroprotective markers, including Brain-derived Neurotrophic Factor (BDNF) and Myelin Protein Zero (MPZ), with β-actin as a reference. The combination of PBM and Insulin produced the most pronounced improvements, demonstrating enhanced cognitive performance, reduced neuropathic pain, and preservation of neuronal structure, peripheral nerve integrity, and multi-organ morphology, including the brain, sciatic nerve, heart, liver, kidney, and lungs. PBM or Insulin alone provided partial protection, with moderate amelioration of functional deficits and tissue pathology. Mechanistically, combination therapy upregulated BDNF and MPZ expression, suggesting a molecular basis for enhanced neuroprotection and remyelination. These findings highlight the potential of PBM combined with Insulin as a synergistic intervention to mitigate diabetes-induced neuropathy and cognitive decline. Incorporating such multi-modal strategies may offer a promising avenue for managing diabetes-related multi-organ complications and improving overall outcomes in diabetic patients.
650			_aMS Biomedical Sciences (BMS)
700			_aSupervisor : Dr. Aneeqa Noor _9119519
856			_uhttp://10.250.8.41:8080/xmlui/handle/123456789/55406
942			_2ddc _cTHE
999			_c615204 _d615204