000			03075nam a22001577a 4500
082			_a610
100			_aPerveen, Anita _9133977
245			_aPsoriasis & Neuroinflammation: Exploring Cytokine Release in Barrier and Metabolic Organs /
264			_aIslamabad : _bSMME- NUST; _c2026.
300			_a99p. _bIslamabad : SMME- NUST; Soft Copy _c30cm
500			_aPsoriasis is a chronic systemic inflammatory disease with its effects beyond skin. One major comorbidity reported to be highly prevalent in psoriatic patients is cognitive impairment. Moreover, overexpression of pro-inflammatory cytokines plays a central role in development and progression of psoriasis and neuroinflammation. This study aims to explore the role of inflammatory cytokines in neurodegeneration and disease progression in psoriatic patients. Furthermore, it assesses the therapeutic effect of curcumin-chamomile based plant extract loaded onto PVA nanoparticles via wet lab and bioinformatics tools. Curcumin and Chamomile Plant extract-based drug was designed and loaded with Nanoparticles to use as treatment. The drug was tested on various parameters to check its toxicity and reactivity before using in-vivo. Psoriatic animal model was designed by imiquimod (IMQ) application on male BALB/c mice. The complete In-Vivo study from disease induction to treatment followed by collection of cortex, hippocampus, Eyes, Skin and Liver was done which were later used for gene expression analysis. Protein hallmarks of neurodegeneration; α-Syn, and inflammatory cytokines like IL‐1β, TNF‐α, PEBP-1 and HSP-90AB1 were measured at transcriptional level in cortex, hippocampus, Eyes, Skin and Liver. Disease induction using IMQ resulted in excessive production of keratinocytes. Pro-inflammatory cytokines were over-expressed whereas α-Syn showed a moderate increase in hippocampus, eyes, cortex, liver and skin. Both treatment groups showed a significant decline in α-Syn and normalization and downregulation of pro-inflammatory cytokines. Molecular docking revealed potential interactions between proteins and cytokines, and proteins and phytochemicals exhibiting potential roles in alleviating the disease pathology. Pathway enrichment analysis was carried out to understand the underlying mechanisms involved in disease progression and roles of these proteins. The increased expression of inflammatory markers causes neuroinflammation and contribute to depression and cognitive impairment, which can be reversed using the novel designed drug. In psoriatic conditions, elevated levels of inflammatory cytokines cause chronic inflammation, which trigger neuroinflammatory transcription factors. The Curcuminchamomile based novel drug encapsulated with nanoparticles comes as a new promising approach to cure neurodegenerative diseases, and the comorbidities arising from psoriasis.
650			_aMS Biomedical Sciences (BMS)
700			_aSupervisor : Dr. Saima Zafar _9122351
856			_uhttp://10.250.8.41:8080/xmlui/handle/123456789/57525
942			_2ddc _cTHE
999			_c616895 _d616895